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Late-development tadpole extract preparation

MC Michael R. Crossland
AS Angela A. Salim
RC Robert J. Capon
RS Richard Shine
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Mid to late developmental stage tadpoles were collected live from the wild (Northern Territory, December 2010), and stored at − 18 °C until extraction. Frozen tadpoles were freeze-dried to give dry material (13.4 g) that was extracted overnight at room temperature with 90:10 MeOH:H2O (100 mL). The resulting solvent extract was concentrated in vacuo at 40 °C to give a crude material (6899 mg), which was partitioned into n-BuOH and H2O solubles. The BuOH extract (3885 mg) containing mostly bufagenins (Fig. ​(Fig.1d)1d) was used in the attractant assay without further purification.

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