Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Heat-killed and glutaraldehyde-fixed green fluorescent protein–Pa or FITC-labeled E. coli (Life Technologies) were incubated for 2 hours (for THP-1 cells, mBMDMs, and mPMs) or 6 hours (for mAMs) at various bacteria:macrophage ratios in 96-well plates. The cells were washed, incubated for 1 minute with 0.4% trypan blue to quench extracellular fluorescence, and washed again, and intracellular fluorescence intensity was quantified by fluorometry.
Copyright and License information: ©2016 by the American Thoracic Society
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
