Reagents and antibodies.

Chemical reagents were purchased from Sigma unless otherwise noted. The gt 3 Kernow-C1/p6 strain of HEV infectious clones was described previously (27). Lalistat 2 (an inhibitor of the lysosomal acid lipase) was a gift from Paul Helquist (University of Notre Dame). Rabbit anti-pORF2 antibody was a gift from X. J. Meng (Virginia Tech). Other antibodies obtained include the following: Rab5A and Rab7A (376801 and 6706-1; Epitomics), clathrin-1 heavy chain (ab21679; Abcam), NPC1 (ab134113; Abcam), dynamin-2 (A303-513A; Bethyl Laboratories), caveolin-1 (ab2910; Abcam), GFP (632375; Clontech), and β-actin (A2228; Sigma). Alexa Fluor-labeled reagents, including transferrin (T-13342) and cholera toxin B subunit (C-34777), were purchased from Invitrogen.