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Immunization With NP-CGG (Nitrophenyl-Chicken Gamma Globulin)

SI Sayaka Ishihara
TS Tsuyoshi Sato
RS Risa Sugioka
RM Ryota Miwa
HS Haruka Saito
RS Ryota Sato
HF Hidehiro Fukuyama
AN Akihiko Nakajima
SS Satoshi Sawai
AK Ai Kotani
KK Koko Katagiri
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WT and DKO mice were intrapenitoneally injected with 100-200 µg of NP-CGG emulsified in complete Freund’s adjuvant (Sigma-Aldrich). The spleens from day 14 and 20 immunized WT and DKO mice were frozen, and the crystal sections were stained with the indicated antibodies. Sera were diluted and analyzed by ELISA using microplates coated with NP30 or NP11-BSA (4-Hydroxy-3- nitrophenylacetyl hapten conjugated to Bovine Serum Albumin) and NP-specific Ig isotypes using Clonotyping System (Southern Biotechnology Association).

Copyright and License information:  ©2021 Ishihara, Sato, Sugioka, Miwa, Saito, Sato, Fukuyama, Nakajima, Sawai, Kotani and Katagiri
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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