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Purified B cells or B-ps were plated into 96-well plates in triplicates and stimulated with 2 and 10 µg/ml goat-mouse IgM F(a)2 or 5 ng/ml IL-7 for 24-48 h. A total of 1mCi [3H] thymidine (GE health Life Science) was added 6h before harvest. Labeled DNA from cells was collected on GSC filters (Whatman), and the radioactivity was measured in a scintillation counter.
Copyright and License information: ©2021 Ishihara, Sato, Sugioka, Miwa, Saito, Sato, Fukuyama, Nakajima, Sawai, Kotani and Katagiri
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