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STRING enrichment analysis

CS Carlos G. Sanchez
CA Christopher M. Acker
AG Audrey Gray
MV Malini Varadarajan
CS Cheng Song
NC Nadire R. Cochran
SP Steven Paula
AL Alicia Lindeman
SA Shaojian An
GM Gregory McAllister
JA John Alford
JR John Reece-Hoyes
CR Carsten Russ
LC Lucas Craig
KC Ketthsy Capre
CD Christian Doherty
GH Gregory R. Hoffman
SL Sarah J. Luchansky
MP Manuela Polydoro
RD Ricardo Dolmetsch
FE Fiona Elwood
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STRING was used to determine statistical enrichment of pathways in gene sets generated from the screens31. Two separate gene sets for this analysis were compiled from gene knockouts that downregulated tau and gene knockouts, that upregulated tau from at least two of the mini pool custom array validation screens with an RSA score cut-off of ≤−5. STRING network edge parameters were set at high confidence (0.700) and are represented by the line thickness between nodes. The gene sets were analyzed using multiple sources including Reactome, KEGG, Gene Ontology, and published literature. Networks were determined based on low false discovery rate (<1.2%) and confidence between nodes ranging from high to highest (0.700-0.900) (Supplementary Data 4 and 5).

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