4.8.1. Preparation of Kidney Tissue Homogenate

The right kidney was excised and rinsed in 0.1 M Tris-HCl buffer (pH 7.4). Ten percent (10%) tissue homogenate was prepared in the same buffer and centrifuged at 1000× g for 20 min using a refrigerated centrifuge (Eppendorf, Taufkirchen, Germany). The supernatant was collected for determination of SOD, CAT, GPx, GST and GR activities, GSH and malondialdehyde levels, and TAC. The results were normalized with protein concentrations of each sample as determined using a commercially available protein assay kit (BioAssay Systems, Hayward, CA, USA). Kidney LDH activity was determined using a commercially available LDH colorimetric assay kit (BioAssay Systems, Hayward, CA, USA).