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4.4. Measurement of Transendothelial Electrical Resistance (TEER)

MK Mihály Kozma
ÁM Ádám Mészáros
ÁN Ádám Nyúl-Tóth
KM Kinga Molnár
LC Laura Costea
ZH Zsófia Hernádi
CF Csilla Fazakas
AF Attila E. Farkas
IW Imola Wilhelm
IK István A. Krizbai
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PBECs were plated into fibronectin/collagen-coated filter inserts of 12 mm diameter (Costar-Corning Transwell Clear, pore size: 0.4 µm). After reaching confluency, endothelial monolayers were supplied with 550 nM hydrocortisone, 250 μM CPT-cAMP and 17.5 μM RO-201724 and placed into the CellZscope instrument (nanoAnalytics, Münster, Germany). TEER was followed continuously. Treatments were applied after approx. 24 h, when TEER reached plateau. TEER was further monitored for 48 h.

Copyright and License information:  ©2021 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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