3.2. Methods

The NMR spectra were recorded on a Bruker 600 MHz spectrometer, equipped with a 5 mm broadband CryoProbe Prodigy. The acquisition parameters are: 90° pulse calibrated at 12 μs, 1.3 s acquisition time, 2 s time of relaxation, no spinning, 300 K, and 2048 scans. The proton NMR spectra were obtained at Forschungszentrum Jülich (Juelich, Germany). The FT-IR spectrum was recorded using FT-IR spectrometry, Nicolet iS5, iD3 with ATR (Thermo Scientific, Japan). Thermogravimetric measurements were carried out using the TGA, Q50 V20.10, Build 36 instrument at a heating rate of 10 °C/min under N₂ gas. The polymer morphologies were investigated using scanning electron microscopy (SEM, Tubney Woods, JEOL 7400F Oxford Instruments Inca, Abingdon, Oxon OX13 5QX, UK).

High Performance Liquid Chromatography (HPLC) was carried out on Waters 1525 Binary HPLC Pump with Photodiode Array Detector PAD (Waters 2998, USA). Data were analyzed using Breeze QS software with the X TERRA C18 column, 5 μm, 250 mm × 4.6 mm, at a flow rate of 1 m/min. The mobile phase was a combination of 36% distilled water and 64% acetonitrile.