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To determine the nuclear accumulation of Nrf2, samples were prepared according to the manufacturer’s protocol using the nuclear extract kit (Active Motif). The TransAM Nrf2 ELISA kit (Active Motif, Carlsbad, CA, USA) was used to quantify Nrf2 in nuclear preparations (10 μg), with absorbance values read at 450 nm of the wavelength.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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