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4.2. Circular Dichroism Spectroscopy
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Circular dichroism (CD) spectra of peptides were recorded on a Jasco J-815 spectropolarimeter (Jasco Int. Co., Ltd., Tokyo, Japan). The CD spectra were acquired for peptides in a concentration of 0.15 mg/mL in 10 mM PBS buffer (pH 7.0) or 30% (v/v) 2,2,2-trifluoroethanol (TFE) in 0.1 cm path length cuvettes and were measured in 185–250 nm spectral range at 25 °C. Ellipticity was measured in mdeg units. The spectra were averaged after three scans.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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