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Food intake and energy expenditure measurement

DS Dollada Srisai
TY Terry C. Yin
AL Abigail A. Lee
AR Alix A. J. Rouault
NP Nicole A. Pearson
JG Justin L. Grobe
JS Julien A. Sebag
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For measurements of cumulative food intake, mice were individually housed in standard cages and, after 7 days of acclimation, food consumption was measured daily at 10.00 a.m. For energy expenditure, food intake, and activity measurements, 7-week-old male mice were individually housed in the home cage for 1 week before housed in CLAMS center feeder cages for 5 days. Data such as food intake, oxygen consumption, heat, and locomotion were collected automatically over a 5-day period and then analyzed with the Oxymax/CLAMS software. Daily food intake and activity data over the experimental period were collapsed to generate average daily food consumption and activity. Heat production was analyzed using analysis of covariance (ANCOVA).

Copyright and License information: The Author(s) ©2017
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