2.4. Statistical Analyses

Data analyses used Matlab (Matlab 2018b, MathWorks, Natick, MA, USA) and IBM SPSS Statistics^® Version 27 (IBM, Armonk, NY, USA). The MK and MD values for integrated molecular glioma subgroups and healthy control groups were displayed on scatter plots. They were then compared to each other visually. We identified a tumor-specific combination of MK and MD values on the scatter plot in the glioma group, which does not occur in other brain or intracranial compartments and goes beyond the healthy brains’ MK and MD values of the control groups.

The corresponding voxels from the scatter plots were labeled and highlighted both on the DKI parametric maps and on the anatomic FLAIR images. Reversely, we compared the automatically labeled voxels with those of the manually segmented tumor volume, and the Dice similarity coefficient was then determined to investigate their spatial overlap.