2.3. Preparation of the Artificial Cerebrospinal Fluid (CSF)

To find further evidence supporting the employability of the BIONOTE system for CSF analysis, an artificial CSF was prepared.

The artificial CSF was freshly prepared before each experimental session dissolving in bi-distilled water the compounds reported in Table 2 [18,19].

Chemical composition of the artificial cerebrospinal liquid.

Next, dopamine and NaHS were added at different concentrations, mimicking some alterations occurring in NDs [20] (Table 3).

Artificial modified-cerebrospinal fluid (CSF) standard concentration (mM) at different concentrations.

To prevent undesirable oxidation phenomena of dopamine, which may potentially affect the voltammetric analysis, each solution was bubbled with nitrogen before dopamine addition [8].