4.6. ATP Assay Detection

ATP Assay Kit (S0026; Beyotime). The cells were treated with different concentrations of 8a (0, 1, 2, 3, 5, and 10 μM) for 30 h, and a certain proportion of cell lysate was added to the cells to make them fully lysed. Then, the cells were centrifuged at 12,000× g at 4 °C for 5 min, and the supernatant was extracted. We added 100 μL of ATP test solution to the 96-well plate and left it at room temperature for 3–5 min. Then, we added 20 μL of sample or standard to the well, mixed quickly, waited at least 2 s, and measured by Luminometer.