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Sertoli cell lines and Cell culture

XC Xiaochun Chi
WL Weiwei Luo
JS Jiagui Song
BL Bing Li
TS Tiantian Su
MY Miao Yu
TW Tianzhuo Wang
ZW Zhenbin Wang
CL Cheng Liu
ZL Zhen Li
HH Huiying He
JZ Jun Zhan
HZ Hongquan Zhang
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Sertoli cell lines 15P-1 and TM4 were purchased from Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (http://www.crcpumc.com/). TM4 cells were cultured at 37 °C with 5% CO2 in DMEM/F12 containing 2.5% heat-inactivated fetal bovine serum (FBS) and 5% horse bovine serum (HBS). 15P-1 cells are derived from testicular cells of PyLT transgenic mice, and have properties of Sertoli cells. 15P-1 cells were maintained in DMEM with 10% heat-inactivated FBS and cultured at 32 °C with 5% CO2. Cells used in this study were purchased from ATCC. We tested the mycoplasma contamination by PCR during the culture processes and all the cells used here were mycoplasma free.

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