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Cytotoxicity/phagocytosis (antibody dependent cell-mediated cytotoxicity/ADCP) assay and Basophil activation test
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Antibody dependent cell-mediated cytotoxicity/phagocytosis (ADCC)/ADCP assays were performed according to a previously described method.14 The ability of SF-25 IgE to trigger primary human basophil activation was determined using an ex vivo assay in which CD63 expression on the surface of human basophils was used as an early marker of basophil activation, as described previously (see online supplemental materials and methods).15
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