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Proximity ligation-assisted ChIP-seq (PLAC-seq)

TW Tianyi Wang
HZ Haijian Zhang
YZ Youlang Zhou
JS Jiahai Shi
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PLAC-seq is a fast, sensitive and economical method to map long-distance chromatin interactions in mammalian cells. It is mainly used to analyse the interactions between different chromatin regions. The principle of the technique is that cells are fixed with formaldehyde and digested. The biotin-labelled nucleotides are then filled in and connected in situ. After lysis of the nucleus, the chromatin is sheared. The soluble chromatin fraction is then immunoprecipitated with specific antibodies modified by anti-transcription factors or histones. Finally, the biotin-labelled DNA corresponding to the linker is enriched, and library preparation and paired-end DNA sequencing are performed [101, 102]. Researchers have used PLAC-seq to map the genome-wide 3D chromatin interactions anchored at DNA bound by histones with H3K27ac modification [29] (Fig. 2D).

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