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Optimization of Substrate Passivation

CP Cas van der Putten
AB Antonetta B. C. Buskermolen
MW Maike Werner
HB Hannah F. M. Brouwer
PB Paul A. A. Bartels
PD Patricia Y. W. Dankers
CB Carlijn V. C. Bouten
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Several passivation protocols were evaluated to optimize the quality of the passivation layer on PDMS substrates as well as the quality of the patterned protein after protein incubation (Table 1). 3-Aminopropyltriethoxysilane (APTES; A3648, Sigma-Aldrich) and poly-l-lysine (PLL, P4707, Sigma-Aldrich) were used in combination with mPEG-succinimidyl valerate (mPEG-SVA; MW 5000 Da, Laysan Bio) as well as Pluronic F-127 (P2443, Sigma-Aldrich) to passivate the PDMS substrates in various ways.

Copyright and License information:  ©2021 The Authors. Published by American Chemical Society
Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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