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Cytokine Multiplex Assay

TL Thatcher B. Ladd
JJ James A. Johnson, Jr.
CM Christen L. Mumaw
HG Hendrik J. Greve
XX Xiaoling Xuei
ES Ed Simpson
MB Mark A. Barnes
BG Brett J. Green
TC Tara L. Croston
CA Chandrama Ahmed
AL Angela Lemons
DB Donald H. Beezhold
MB Michelle L. Block
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Frontal lobe tissue was prepared as specified by the Milliplex Multiplex Luminex assay manufacturer instructions. Specifically, 100 µg of protein was assessed for changes in pro-inflammatory cytokines using the Mouse High Sensitivity T Cell Milliplex Multiplex Luminexassay (Millipore Sigma, Merck KGaA, Darmstadt, Germany), following manufacturer instructions. Samples were analyzed by and data were collected from a Bio-Plex 200 system (Bio-Rad) and analyzed with Bio-Plex Manager software (Bio-Rad). Standard, sample, and control wells with bead counts <37 were excluded. The analysis software employed a five parameter logistic regression standard curve to determine sample cytokine levels.

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