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Measurement of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in mouse serum
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Serum was obtained from blood samples on Day 6 p.i. and the levels of AST and ALT measured immediately using Transaminase CII-Test Wako (Wako Chemicals) according to the manufacturer’s instructions. The OD was measured at 555 nm in a Corona Grating Microplate Reader SH-9000 (Corona, Electric Co., Ltd.). The OD values were converted to Karmen units by using AST and ALT calibration curves.
Copyright and License information: ©2016 Phanthanawiboon et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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