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FITC-dextran gut permeability assay
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Mice were induced with two injections of 3 mg (120 mg/kg) and 2 mg (80 mg/kg) tamoxifen on consecutive days. On day 7 post induction, mice were fasted for 12 h and then dosed with 600 mg/kg FITC-dextran (4 kDa; Sigma, #46944) by oral gavage. Mice were sacrificed and blood was sampled 4 h post gavage. Serum concentrations of FITC-dextran were determined using a fluorimeter (490/530 nm) against standard curves generated from the same stock solution used to dose the mice.
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