Drugs and administration

A racemic mixture of (±)-propranolol hydrochloride (#P0884) and (–)-EGCG (#E4268) were obtained from Sigma-Aldrich. Animals of three months of age were treated with either vehicle (saline solution), propranolol (10 mg/Kg), or EGCG (20 mg/Kg) by intraperitoneal administration in a volume of 100 μl, three times per week, during two months. Drug doses were based on a bibliographic search for both propranolol (38,45) and EGCG (43). These studies concluded that the doses we used in our study were safe and that both compounds reached the brain to exert their effects.