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Animal model design

KH Keyu Huang
CY Chunqing Yang
JZ Jian Zheng
XL Xiaobai Liu
JL Jie Liu
DC Dongfang Che
YX Yixue Xue
PA Ping An
DW Di Wang
XR Xuelei Ruan
BY Bo Yu
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Adult male C57BL/6J mice aged 12–14 weeks were used to make CCI models. All experiments were approved by the Ethics Committee of China Medical University. ARRIVE guidelines were followed. The mice were individually housed in an environment with suitable humidity and temperature and were given standard diet. In order to identify the effect of CCI on the hippocampal neurons in mice, 24–29 grams of mice were randomly divided into two groups: sham operation group (n = 15) and CCI group (n = 20). In order to observe the effect of circ_0000296 on hippocampal neuron apoptosis, the mice weighing 24–29 g were randomly divided into three groups: CCI group (n = 20), CCI + circ_0000296(+)-NC group (n = 20), CCI + circ_0000296(+) group (n = 15).

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