EdU incorporation, staining and TUNEL assays

Gli1-CreER;Arid1a^fl/fl mice and littermate controls were injected with EdU (10 μg/g body weight) intraperitoneally 48 hours before being euthanized. The mandibles were fixed and decalcified. Click-iT plus EdU cell proliferation kit (Thermo Fisher, {"type":"entrez-nucleotide","attrs":{"text":"C10637","term_id":"1535708","term_text":"C10637"}}C10637) was used on the cryosections for in situ EdU detection according to the manufacturer’s instructions.

Cell apoptosis was detected using a TUNEL assay (Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection, Thermo Fisher, {"type":"entrez-nucleotide","attrs":{"text":"C10617","term_id":"1535688","term_text":"C10617"}}C10617) according to the manufacturer’s protocol.