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Liquid chromatography mass spectrometry bile acid quantitation in bile.

TG Trisha J. Grevengoed
ST Samuel A.J. Trammell
JS Jens S. Svenningsen
MM Mikhail V. Makarov
TN Thomas Svava Nielsen
JJ Jens Christian Brings Jacobsen
JT Jonas T. Treebak
PC Philip C. Calder
MM Marie E. Migaud
BC Benjamin F. Cravatt
MG Matthew P. Gillum
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Bile diluted 1/400th methanol was diluted to either 1/1200th or 1/36,000th in a Phenomenex Verex 9 mm vial containing internal standard solution (0.3 μM of D4 taurolithocholic acid-d4 [TLCA-d4], D4 taurodeoxycholic acid-d4 [TDCA-d4], D6 deoxycholic acid-d6 [DCA-d6], D5 cholic acid-d5 [CA-d5], D4 taurocholic acid-d4 [TCA-d4], D4 tauroursodeoxycholic acid-d4 [TUDCA-d4], D5 lithocholic acid-d5 [LCA-d5], and D4 chenodeoxycholic acid-d4 [CDCA-d4]) in water. Steroidal bile acids were detected and quantified as previously described (39).

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