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Assessment of Cell Membrane Integrity Using Propidium Iodide
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Propidium iodide (PI) is an impermeable nucleic acid dye that emits bright red fluorescence when it binds to DNA and RNA following cell membrane injury.
Following HR, the cells were stained with 200 μL of PI (1 mg/ml solution of PI diluted 1:3,000 in PBS) for 30 min, washed extensively in PBS for 3 min, fixed with 4% paraformaldehyde for 20 min, counterstained with 4′,6-diamidino-2-phenylindole (DAPI) to identify nuclei, and imaged using a fluorescence microscope.
Copyright and License information: ©2021 Tang, Liao, Pao, Hsu, Wu, Huang and Chu.
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