Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
pDestTol2pACryGFP-4xnrUAS-nCas9n (4xUAS:NLS-Cas9,cryaa:EGFP, plasmid map in Fig. S7B) was constructed with entry vectors p5E-4xnrUAS, pME-nCas9n and p3E-polyA and destination vector pDestTol2pACryGFP (Addgene plasmid #64022, deposited by Joachim Berger) by LR cloning reaction. All plasmid sequences are available upon request.
Copyright and License information: ©2021 2021. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
