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2.8. Cytopathic effect (CPE) based SARS-CoV-2 Neutralization assay

PK Praveen Kumar
RP Reshma Perween
TS Tripti Shrivastava
VS Vanshika Singh
HP Hilal Ahmed Parray
SS Swarandeep Singh
AC Adarsh Chiranjivi
RT Ramachandran Thiruvengadam
SS Savita Singh
NY Naveen Yadav
KJ Kamini Jakhar
SS Sudipta Sonar
SM Shailendra Mani
SB Sankar Bhattacharyya
CS Chandresh Sharma
PV Preeti Vishwakarma
RK Ritika Khatri
AP Anil Kumar Panchal
SD Supratik Das
SA Shubbir Ahmed
SS Sweety Samal
PK Pallavi Kshetrapal
SB Shinjini Bhatnagar
KL Kalpana Luthra
RK Rajesh Kumar
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CPE based neutralization assays were performed as described previously in Parray et al., 2020 [14]. Briefly, 1 × 102 TCID50 isolate; USA-WA1/2020 virus, passaged once in Vero cells, was incubated with serum dilution ranging from 1:20 to 3260 for 90 mins, followed by 1 h of adsorption on the Vero cells. After washing the cells, DMEM supplemented with 2% (vol/vol) FBS was added. The presence of cytopathic effect (CPE) in cells was detected using a microscope after incubation for 4-5 days at 37°C with 5% CO2. Non-infected VERO E6 cells were used as a positive control, and infected VERO E6 cells were used as a negative control.

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