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For MTT assay, MDA-MB-231 and BT-549 cells were digested, collected, and seeded in 96-well plated at a density of 2×104 cells per well and were added to culture medium and incubated for 24 and 48 hours. At indicated time point, MTT reagent was added into each well and incubated for another 4 hours. At last, cell culture medium was discarded and 150 μL DMSO was added to incubate in dark for 15 minutes. The absorbance values at 570nm were detected by a microplate reader (Bio-Tek EL 800, USA).
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