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2.6. Tumor Tissue Explants Culture, Preparation of Formalin-Fixed Paraffin-Embedded (FFPE) Blocks, and Tissue Microarray

WS Walid Shalata
MI Muhammed Iraqi
BB Baisali Bhattacharya
VF Vered Fuchs
LR Laila C. Roisman
AC Ahron Yehonatan Cohen
IM Ismaell Massalha
AY Alexander Yakobson
MP Manu Prasad
ME Moshe Elkabets
AP Angel Porgador
NP Nir Peled
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The PDXs were used for preparation of 2 × 2 × 2 mm3 ex vivo tumor tissue explants culture according to a previously published protocol [11]. FFPE blocks were then prepared from the explants using an automated tissue processing machine (Leica Biosystems, Nußloch, Germany) [11,12,13]. Finally, tissue microarray (TMA) blocks were prepared from donor FFPE blocks using 3-mm T-SueTM punch needles (Simport, Beloeil, QC, Canada), with each block containing up to 24 tissue explants.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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