2.6. Tail Suspension Test and Forced Swim Test

Depressive-like behaviors were evaluated in the TST [36] or the FST [37], two standard procedures used to evaluate the antidepressant activity of pharmacological compounds. In TST, mice were suspended at a height of 20 cm above the floor and are surrounded by an enclosure and the total duration of immobility (passive hanging) between periods of wriggling to avoid aversive situation was measured for a period of 6 min, and the latency to the first immobility was also noted, as previously described [38]. In FST, mice were placed for 6 min into a cylinder (17 cm in diameter) filled with 25 °C tap water (at a height of 13 cm) with no way out. The immobility duration (excluding movement necessary to keep the head above water or to float) was measured as an indication of the behavioral despair of mice [38].