2.8.2. Ferric Chloride-Induced Arterial Thrombosis Model

After an hour, rats were anesthetized with 2% sodium pentobarbital (0.25 mL/100 g.bw). The neck skin was removed, and the muscle layer was cut open along the midline. Avoiding lymphatic and glands, the left carotid artery was separated about 1 cm. The carotid artery was encircled with a filter paper strip (1 cm × 0.5 cm) soaked with 10% FeCl₃ solution, then the wound was covered with gauze soaked with normal saline and kept for 15 min. After that, the filter paper strip was removed and kept for 40 min. After clamping the two ends of the blood vessel with hemostatic tweezers, blood was taken from the abdominal aorta [32,33]. After blood collection, the blood was mixed with 3.8% sodium citrate (9:1, v/v) for anticoagulation. After gently mixed, the blood was centrifuged for 15 min at 1500× g. The upper light-yellow plasma was collected and stored at −80 °C for later coagulation function test. The blood vessel segment was cut off, about 1 cm wrapped in filter paper strip, the residual blood inside was absorbed with filter papers, the overall wet weight was weighed, and the blood vessel was weighed after taking out the thrombus. After the rats were sacrificed, the thymuses and spleens were collected and weighed (mg) to obtain the thymus index and spleen index.