2.3. Hematoxylin and Eosin (H&E) Staining and Histological Analysis

The brain tissues collected from the rats were fixed in 4% paraformaldehyde at 4 °C for 24 h, and the cerebral cortices were cut into 3 mm-thick sections. The samples were dehydrated in graded solutions of ethanol, immersed in xylol, embedded in paraffin, and sectioned to a thickness of 4 μm. The obtained tissue sections were assembled on glass slides and stained with H&E. All samples were viewed and photographed under a Leica light microscope equipped with a digital camera (DMI3000B, Leica, Germany).