2.7.2. Determination of Catalase Activity

Sinha’s assay evaluates catalase (CAT) activity with minor adaptations [78]. CAT acts on hydrogen peroxide for a well-defined period, after which it is inactivated by adding a mixture of potassium dichromate-acetic acid. After CAT inactivation, the amount of unmodified oxygenated water was reduced in the acid medium, the potassium dichromate, to chromic acetate, which was determined at 570 nm. The difference between the initial and final quantity of oxygenated water in the reaction medium constitutes the amount of oxygenated water decomposed by catalase.