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Negative Geotaxis Assay

MD Meng Ding
LZ Lan Zheng
QL Qiu Fang Li
WW Wan Li Wang
WP Wan Da Peng
MZ Meng Zhou
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According to the previous method (Birse and Bodmer, 2011). The climbing apparatus consisted of an 18-cm-long glass tube with an inner diameter of 2.8 cm (sponges were placed in the ends of the tube to prevent escape yet allow air exchange). Due to the instinctive negative geotaxis displayed by Drosophila, the flies climbed up the sides of the apparatus after being taped down at the bottom. Flies were allowed 8 s to climb after being taped down, and the climbing heights reached by the flies were calculated. A video camera was used to film the fly climbing during the whole process, the 4th, 5th, and 6th climbing images at the end of 8 s were intercepted, and the number of flies reaching the top was counted. Climbing index = number of in the uppermost area/total number.

Copyright and License information:  ©2021 Ding, Zheng, Li, Wang, Peng and Zhou.
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