Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
IHC-labeled samples were immersed in a mixture of 1:1 CUBIC2 solution and PBS. The well plate was sealed with Parafilm then placed in a shaking waterbath at 37°C for 3h. The CUBIC2/water solution was then discarded and replaced with 100% CUBIC2 solution and kept at 37°C at least overnight, but maximum 72h. The day before imaging, samples were placed in a 1:1 mixture of mineral oil (Sigma - M8410) and silicone oil (Sigma - 175633).
Copyright and License information: ©2021 The Authors
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
