Fresh fecal samples from each group of mice were collected daily after infection to determine the prepatent period (malachite green stain), and the experiments were terminated when no Cryptosporidium oocysts could be detected under the microscope. Cryptosporidium oocysts were isolated from fecal samples using Sheather’s sugar floatation method [17], and the number of oocysts per gram (OPG) of feces was counted using a hemocytometer slide. Each group sampling was repeated three times to calculate an average value. Number of OPG was estimated according to the number of oocysts counted and a curve diagram of oocyst excretion [26].
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