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The concentration of every sample was determined using the Qubit High Sensitivity DNA kit (Life Technologies). If necessary, the samples were diluted to 1.8 ng µl−1 and the fragment length distribution was evaluated on a Bioanalyzer High Sensitivity dsDNA chip (Agilent Technologies). Adapter dimers are expected to show fragment lengths of around 125 bp, whereas libraries with miRNA insert should show fragment lengths of around 145 bp.
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