The shredded fresh sugarcane samples were immediately collected and inactivated at 100 °C for 1 h to denature and deactivate the enzymes, as well as to prevent sugar degradation by microbial. Subsequently, the inactivated samples were dried under 60 °C until no loss of weight. The dried samples were ground over 40 mesh for offline NIRS spectrum data collection and further sugar content analysis. MATRIX-F equipped with a Q413 sensor head was used for contactless offline measurements. The reflectance of each sample was recorded and averaged for further calibration analysis.
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