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Methylation quantitative trait loci (meQTL) analysis

KB Karin Bolin
JI Juliana Imgenberg-Kreuz
DL Dag Leonard
JS Johanna K. Sandling
AA Andrei Alexsson
PP Pascal Pucholt
MH Malena Loberg Haarhaus
JA Jonas Carlsson Almlöf
JN Joanne Nititham
AJ Andreas Jönsen
CS Christopher Sjöwall
AB Anders A. Bengtsson
SR Solbritt Rantapää-Dahlqvist
ES Elisabet Svenungsson
IG Iva Gunnarsson
AS Ann-Christine Syvänen
KL Karoline Lerang
AT Anne Troldborg
AV Anne Voss
ØM Øyvind Molberg
SJ Søren Jacobsen
LC Lindsey Criswell
LR Lars Rönnblom
GN Gunnel Nordmark
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We performed a cis-meQTL analysis investigating DNA methylation levels in whole blood from LN patients from the discovery cohort (n = 180 with DNA methylation data available) against the genotypes of SNPs with a nominal p-value <0.001 in LN versus SLE without nephritis analysis in the discovery cohort (n = 110 SNPs) and SNPs in genes that had been previously associated to LN (n = 482, n = 592 SNPs in total) [6]. DNA methylation data were generated on the HM450k methylation array, normalized and quality controlled as previously described [11]. All CpG sites located within a 100 kb flanking region of these SNPs were included, and methylation levels were tested in PLINK for genotype association in LN patients assuming an additive model.

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