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Intraperitoneal glucose tolerance test (IPGTT)
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After 5 weeks of treatment with MC, IPGTT was conducted in 6-h fasted mice as previously described (Naowaboot et al., 2016a). Mice were injected intraperitoneally with 2.0 g/kg of glucose, and blood glucose was measured at 0, 20, 60, and 120 min. The area under the curve (AUC) of blood glucose over the time was calculated using trapezoidal analysis.
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