2.10.2. Shake flask method (dynamic test)

The antimicrobial activity of film samples was also studied according to the shake flask method. In this method, bacteria and mold grown in 100 ml of Mueller Hinton Broth were used. 2 × 10⁵ CFU/ml of each microorganism were added to 100 ml of Mueller Hinton Broth medium and incubated at 37℃ for 12 hr. Every 2 hr, the absorbance was recorded by spectrophotometer UV/Visible (Shimadzu, model UV‐1700, Japan) at a wavelength of 600 nm, and microbial growth was obtained. The test was repeated 3 times, and the average values were used (Sadeghnejad et al., ²⁰¹⁴).