Protocol to request
            
                Generation of endogenously tagged cell lines
                
                
            
        
        
            
                Research article
            
            
                SAC1 degrades its lipid substrate PtdIns4P in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes
                
            
            
            
                James P Zewe et al.
            
            
                DOI: 10.7554/eLife.35588