Request a detailed protocol

As part of our efforts to make science more reproducible, we're working with Bio-protocol to link the protocols they host with research published at eLife. To request a protocol for an eLife paper that's not yet available on Bio-protocol, fill in the form below and they will send a request to the author.

If the author chooses to make it available, the Bio-protocol team will let you know via email and a link will be added to the eLife article. Learn more about requesting a protocol.

Please do not ask authors to share any details that are already available in the paper.

Protocol to request

Isolation and culture of primary astrocytes

Research Article

Upregulated expression of ubiquitin ligase TRIM21 promotes PKM2 nuclear translocation and astrocyte activation in experimental autoimmune encephalomyelitis
Luting Yang et al.

DOI: 10.7554/eLife.98181

Your name

Please enter your name

Your email address

Please enter your email address

Your message to authors Tips for writing messages

Please include a message to authors

Post your question anonymously to a public Q&A (optional).

This will add your question to a Q&A on the Bio-protocol journal's sister site, Bio-protocol Exchange, so that others can benefit from the discussion of this protocol.

By sending your request you are consenting to eLife passing your details to Bio-protocol and for Bio-protocol to contact you via email. Your details and message will also be sent to the corresponding author. Please read the privacy policy for more details.