Request a detailed protocol

As part of our efforts to make science more reproducible, we're working with Bio-protocol to link the protocols they host with research published at eLife. To request a protocol for an eLife paper that's not yet available on Bio-protocol, fill in the form below and they will send a request to the author.

If the author chooses to make it available, the Bio-protocol team will let you know via email and a link will be added to the eLife article. Learn more about requesting a protocol.

Please do not request that authors share details that are already available in their research article.

Protocol to request

Total RNA isolation and quantitative real-time PCR (qRT-PCR) analysis for assessment of mRNA expression during pituitary organoid development

Research article

Modeling corticotroph deficiency with pituitary organoids supports the functional role of NFKB2 in human pituitary differentiation
Thi Thom Mac et al.

DOI: 10.7554/eLife.90875

Your name

Please enter your name

Your email address

Please enter your email address

Your message to the authors Tips for writing messages

Please include a message to authors

Post your question anonymously to a public Q&A (optional).

This will add your question to a Q&A on the Bio-protocol journal's sister site, Bio-protocol Exchange, so that others can benefit from the discussion of this protocol.

By sending your request you are consenting to eLife passing your details to Bio-protocol and for Bio-protocol to contact you via email. Your details and message will also be sent to the corresponding author. Please read the privacy policy for more details.