Protocol to request
            
                Image processing, discounting light scatter from neighbors, outlining cells, and deconvolution
                
                
            
        
        
            
                Research Article
            
            
                Spatial and temporal distribution of ribosomes in single cells reveals aging differences between old and new daughters of Escherichia coli
                
            
            
            
                Lin Chao et al.
            
            
                DOI: 10.7554/eLife.89543