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Protocol to request

Overexpression and purification of recombinant Csm5, Csm5Δ46, PNPase, RNase R, and Cbf1 from E. coli

Research Article

Critical roles for ‘housekeeping’ nucleases in type III CRISPR-Cas immunity
Lucy Chou-Zheng et al.

DOI: 10.7554/eLife.81897

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