Protocol to request
            
                Construction of 1D free energy profiles
                
                
            
        
        
            
                Research article
            
            
                Rate-limiting transport of positively charged arginine residues through the Sec-machinery is integral to the mechanism of protein secretion
                
            
            
            
                William J Allen et al.
            
            
                DOI: 10.7554/eLife.77586