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Protocol to request

PARP1 activity as detected by HPLC assays that monitor consumption of NAD⁺, formation of nicotinamide and ADPR, and release of AMP-PR following digestion with phosphodiesterase

Research article

HPF1 and nucleosomes mediate a dramatic switch in activity of PARP1 from polymerase to hydrolase
Johannes Rudolph et al.

DOI: 10.7554/eLife.65773

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